My X-chromosome Inheritance – the hunt for Mr.X

One of my favourite family board games is “Scotland Yard, the Hunt for Mr. X“. In this game you have 24 turns for a team of players to find the Spy (Mr.X) as he moves around the streets of London. I mention this because not only is this a great game, it’s also a rather clumsy, but accurate, way to introduce this blog topic, the X-chromosome.

I’ve been inspired to do this by gedmatch, the vendor-neutral website for comparing your DNA. Normally I’m not a big user of gedmatch, however I was surprised when I checked my X-DNA matches and saw that my top match was an unknown relative with a whopping 47.7cM matching segment on the X chromosome. To put this in context, the whole of the X chromosome is only 195.93 cM long AND my largest autosomal DNA match is only 42.4 cM over the other 22 chromosomes. This match was clearly worth investigating.

X-chromosome for Dummies

Before I go further on my own X-chromosome I think it’s worth giving a brief recap on the X chromosome (hereafter referred to by it’s more typist-friendly name of X). The X is part of the sex-chromosome. It’s what defines you as biologically either male or female. A male has only one, which he receives from his mother, whilst getting the rather puny Y from his father. A female has two Xs, one from their mother and one from their father. There is one other important difference here. The X that a mother gives to her children is recombined during meiosis. I’m afraid I’m rubbish at biology, if you are interested in the topic I’d highly recommend you do some background reading on meiosis and recombination.

In the mean time let me try and give you an executive summary. The X that a mother gives to her children is a mix of the two X chromosomes she has, this is what is referred to as “Recombination. This mean that on average that X should be a mix of 50% of the X she received from her father and 50% of the X she received from her mother BUT it can be, and sometimes is, that she passes the whole of one of her X chromosomes to a child.

As you go back through the generations this process means two important things for a genealogist:

  1. You inherit your X or Xs from a specific subset of your ancestors. Trivia fact: The number of ancestors who a male inherits X chromosome DNA from in each generation follows the Fibonacci Sequence (1,1,2,3,5,8 etc. – assuming that the first 1 is the male’s own X…)
  2. In theory it is likely that you inherit your X in different proportions from different ancestors.

This process makes the whole understanding of X inheritance rather complex. Fortunately there are some nice charts for genealogists to use which help you understand this inheritance. I’ve taken the chart below from this website: www.thegeneticgenealogist.com/2009/01/12/more-x-chromosome-charts. There is obviously a slightly different chart for females, but I’m keeping that out of this blog, since the theory is challenging enough.

X Chromosome inheritance chart for a Male

If I apply this to my family tree, then you can see who could have contributed to my single X chromosome, and what proportion the should have contributed.

Hopefully this is clear, I’ve tried to shade the ancestors so it shows how much/how likely they contributed to my X.

As you can see, if you go back 5 generations there are only 8 people who could have contributed to my single X. Theoretically the largest contribution comes from Mary Barr (25%), whilst the lowest is from William Angus and Elizabeth Singleton who should have contributed only 6.25%. Contrast this with my regular autosomal DNA (the 22 pairs of non-sex DNA chromosomes) when all 32 ancestors should have contributed 3.125%.

There is a fly in the ointment. X chromosomes sometimes don’t seem to re-combine at all. It’s worth reading this article on the That Unruly X by genetic genealogist Roberta Estes. Having read this article I was curious to find out more about my own X.

Finding my X

The first step on my search was to compare my X against my brother. All four major  DNA testers include SNP testing of the X as part of their autosomal testing. The exact number of SNPs they currently test (according to the ISOGG Wiki) are: AncestryDNA (28,892), FamilyTreeDNA (19,487) , 23andMe (18,091), and MyHeritage (17,889). Whilst the numbers differ I do not think there is a significant difference between the results the test companies provide. Since FamilyTreeDNA.com is where my brother and myself tested, and their website offers a chromosome browser I looked at their results first. Below is the result I got from comparing myself against my brother:

X-Chromosome comparison via FamilyTreeDNA. Orange segments are where we match

Remeber here that my brother and myself have only on X each. So logically, every time our Xs make a switch from match to non-matching (and vice-versa) it is the result of a recombination event in one of our Xs. Without further information we don’t know if this was as a result of my X recombining, or my brother’s. If I look at this result it would appear that there are 9 such changes of state, implying 9 recombination events. To confirm this I also checked our results on gedmatch, which has a specific X one-to-one match-checking function. The result there was interesting. There were only 7 recombination events.

To confirm this I then went back to the familytreeDNA website and compared the two kits I have of my own DNA against each other. They should match over the complete length of the X. But the don’t, clearly there is a segment of the X that does not match:

X Chromosome comparison Myself match: Myself (orange) and my brother (light blue)

I can only assume that this is a SNP-poor part of the X that the FamilyTreeDNA matching mechanism cannot handle well. But at least it’s now clear, we are down to seven X recombination events, as the table below (taken from gedmatch) shows:

Chr Start Location End Location Centimorgans (cM) SNPs
X 12,192,147 33,957,251 37.0 3,244
X 45,064,135 114,485,507 47.3 5,216
X 125,242,332 141,847,221 24.2 1,979
X 147,291,278 154,545,424 16.9 1,057

Identifying Recombination events through Visual Phasing

The fun thing is we can now use the incredibly long segment of matching X from my x-match with an unknown shared ancestor to provide us with more information. This process is the same as used in “Visual Phasing“. As genetic genealogist Blaine Bettinger describes it:

“Visual Phasing is a process by which the DNA of three siblings is assigned to each of their four grandparents using identified recombination points, without requiring the testing of either the parents or grandparents”

I must admit I’ve tried to avoid Visual Phasing as a technique because it’s complex AND I don’t have the required number of siblings, or even first cousins that would help here, but in this case the long X match relation will work. Below are three one-to-one X comparisons done via gedmatch. The first “barcode” diagram is my brother and myself.

The second barcode is the X match between myself and my unknown relation. I’ve included the segment positions to make it clearer.

The third comparison: my brother compared to our unknown relation.

These three graphs show two important points:

  • Looking from the start of the X chromosome; I match the unknown relation before and after the point where I first match my brother (position 12,192,147). This means that my X is very unlikely to have recombined at that point. My brother’s did.
  • I stop matching the unknown relation at approximately the same position as I stop matching my brother (position 33,957,251 ). My brother continues to match the unknown relative. This means my X recombined at that point.

As a result I can now identify two segments each for myself and my brother:

Who Start Location End Location Centimorgans (cM) SNPs
Me 0 33,957,251
Me 33,957,251 45,064,135
Brother 0 12,192,147
Brother 12,192,147 45,064,135

As further support for this, I’ve a couple more X comparisons to show. Again this is myself (10.6 cM), then my brother (27.8 cM), compared to a third party, namely WilliamP. Both our shared segments start at the same position (30,187,894). This does not fit with a our recombination event locations, so it must be that WilliamP’s X recombined at that point. You  can see that my, shorter, shared segment finished at the position 33,957,251, which is the exact point I am expecting my X to have recombined.

Completing the X.

Now that I’ve got my first two recombination events decided I want to try and see if I can completely map X. Unfortunately there are no other really large X-matches that I can use to determine recombination points. The good news is that some of the smaller c.20cM matches can help.

This is the first one, labelled NB. Below are the two barcode chart, myself and my brother, both compared to NB:

As you can see both start at approximately the same position around the 98,000,000 location. Whilst my brother’s match continues further, mine stops at 114,579,504, which is roughly the same position as myself and my brother stop matching (114,485,507). This means I must have had a recombination event at this point.

The next X-match that we can look at is one that for my brother hardly exists, whereas I have a 23 cM match. Note: I had to force gedmatch to use lower parameters to solve this part of the X. The matches, with an individual CG, are shown below:

 

My brother only begins to match CG at the point 125,212,114. This is roughly the same position as my brother and I begin to share the same X. In other words he has a recombination event at this point.

Putting this information together we can now identify the following X lengths.

Who Start Location End Location Centimorgans (cM) SNPs
Me 45,064,135 114,485,507  47.3 5,216
Me 114,485,507 141,847,221
Brother 45,064,135 125,242,332
Brother 125,242,332 141,847,221  24.2 1,979

I’d love to be able to say that I found other X matches that allowed me to complete this exercise, however having combed through around 50 top X matches for both myself and my brother there are no other matches that clarify any of the recombination events. After the top 50 matches the matching X lengths are around 10cM or less and so far, none cross the missing recombination points.

Putting it all together

I’ve now discovered whose X DNA recombined at 4 points on the X chromosome. Frustratingly there is no X match that identifies who had a recombination event with me at the other 3 locations. Still it’s interesting to see that both my brother and myself have at least two recombination events each. It is certainly not the case that either of us inherited the whole of one of our Mum’s X chromosomes.

Event Location Who
12,192,147 Brother
33,957,251  Me
45,064,135  Unknown
114,485,507  Me
125,242,332  Brother
141,847,221  Unknown
147,291,278 Unknown

The other outstanding question is matching my X chromosome segments to my two maternal grandparents. This relies on finding a cousin with an X match and an identifiable common ancestor. Ideally I’d have a second cousin from of my Mum’s family with whom I share X. Sadly non of them are currently on gedmatch. The alternative is to resort to an unknown cousin and try and work out their match. This can be tricky as the odd inheritance pattern of  X often results in X matches that share a common ancestor some generations before accurate genealogical records were kept. This is the case with my longest X match, MD, although in this case it looks possible that our match is with the Bell family in Ulster sometime in the eighteenth century. This match fits both our X inheritance patterns and is one of the more likely sources if you look at theoretical percentages of X inheritance.

Final Thoughts

Having reached this point it’s rather frustrating that I cannot complete my X chromosome inheritance, however that is sometimes the case with a genealogical investigation. I have a second cousin who plans to upload here DNA results to gedmatch, at which point I hope to be able to update this blog.

If you are interested in the whole process of Visual Phasing then I would recoomend you join this group on Facebook: www.facebook.com/groups/visualphasing/

No discussion of Visual Phasing is complete if it doesn’t mention the amazing spreadsheet that Excel-developer Steven Fox put together. If you are interested in Visual Phasing then you should try his spreadsheet. You can get hold of this from the Visual Phasing Working Group on Facebook. The magic of this spreadsheet is that it allows you to use Excel columns to align with recombination events from the gedmatch barcode diagrams. As an example, below is my longest X match (MB) compared with myself and my brother:

Visual Phasing Spreadsheet (Speculative)

The olive green and purple lines marked G3 and G4 are where I’ve painted in the Xs to indicate the recombination event locations. It’s a little speculative at the moment, but I’m hoping one day it will be complete and correct.

Advertisements
This entry was posted in Genetic Genealogy, X chromosome, X-chromosome and tagged , , , , , . Bookmark the permalink.

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out / Change )

Twitter picture

You are commenting using your Twitter account. Log Out / Change )

Facebook photo

You are commenting using your Facebook account. Log Out / Change )

Google+ photo

You are commenting using your Google+ account. Log Out / Change )

Connecting to %s